Enhancing Biobutanol Production from biomass willow by pre-removal of water extracts or bark

Aiming to understand the importance of debarking on controlled utilization phenolic-rich willow biomass, biobutanol was produced from it by using Clostridium acetobutylicum. Acid-catalysed steam explosion and enzymatic hydrolysis (EH) were investigated before acetone-butanol-ethanol (ABE) fermentation. The hydrolysable sugar yield ABE fermentation efficiency found decline progressively wood (WW) HWE WB (hot water extracted biomass), (willow biomass) WW + plus artificial bark extracts), indicating that pre-removal extracts or can significantly improve yield. Notably, productivity achieved 12.7 g/L at solvent 31%, butanol concentration (i.e. 8.5 g/L) generated is relatively high among reported lignocellulosic-derived biomass. Additionally, hypothesized under acidic conditions temperatures fructose present in form hydroxymethylfurfural during explosion, which then spontaneously condenses with phenolic substances a solid furanic precipitate. formed precipitates play inhibitory role are thereby deleterious